Production of 4-Ipomeanol, an anticancer agent from the root tubers and rhizogenic callus of Ipomoea batatas Lam.: A Comparative study.

A comparative study was done on the production of 4-ipomeanol from root tubers of Ipomoea batatas and its rhizogenic callus. Best callusing response was obtained on MS medium supplemented with 11 µM NAA (α-Naphthaleneacetic acid) and 1 µM KIN (Kinetin). Effect of various elicitors (Fusarium solani, chitin and chitosan) on the production of 4-ipomeanol was studied. Methanol extract of the samples were purified by column chromatography and detected using TLC. Identification of 4-ipomeanol was confirmed using HPLC and quantified spectrophotometrically. A mass spectrum was recorded to confirm the presence of 4-ipomeanol. The calli grown under chitin produced highest (6.61mg g-1) amount of 4-ipomeanol. This is the first report on in vitro production of 4-ipomeanol from I. batatas. Since 4-ipomeanol is reported to be present only in I. batatas, this study would help in standardizing protocols for large scale production without affecting its natural flora.

[Spasmolytic effect of zataria multiflora boiss leaf extract on rat uterus]

Zataria multiflora Boiss is used in traditional medicine to treat gastrointestinal disorders and menrrhalgia. The inhibitory effect of this herb on rat ileuni contractions has also been reported. The aim of this study was to investigate the effect of Zataria multiflora Boiss hydroalcoholic leaf extract [ZHLE] on isolated rat uterus in the presence of some known uterus stimulants. Pieces of virgin adult rat uterus were mounted in an organ bath containing Tyrode or De Jalon solutions. Uterus contractions were induced by KCI, oxytocin and BaC1[2] in presence and absence of ZHLE. Animals in oxytocin studies received an injection of oestradiol valerate [5mg/kg, s.c.] 24 h prior experiment. ZHLE [0.125, 0.25, 0.5, 1 and 2 mg/ml] relaxed the uterus precontracted by KC1 [60mM] in a dose-dependent manner [p<0.0001] and at 2mg/mi attenuated the BaC1[2] [4mM]-induced uterus contraction significantly [p<0.001]. The inhibitory effect of ZLHE on KC1-induced uterus contraction was unaffected by propranolol [1 micro M]. in normal IDe Jalon solution, ZHLE [0.125, 0.25, 0.5, and 1 mg/ml] reduced the oxytocin [10mU/ml]-induced contraction dose-dependently [p<0.0001] but in Ca[2+]-free Dc Jalon solution, the stimulatory effect of oxytocin was weaker and also the inhibitory effect of ZHLF was more consistent. In presence of atropine [0.5jiM], acetylcholine [0.5jiM] failed to induce contraction but KC1 [30mM]-evoked contraction and extract diminished the contractile response of KC1. The spasmolytic effect of extract [2mg/ml] on KC1-induced contraction was unaffected by naloxone [1 micro M]. From the obtained results it may be concluded that, the ZHLE may induce its inhibitory effect through blockage of the voltage dependent calcium channels and releasing calcium from intracellular stores in rat uterus smooth muscle. The ineffectiveness of propranolol and naloxone on ZLHE inhibitory effect indicates that adrenergic and opoids agonist substance[s] did not exist in the extract. it seems that there was no anticholinergic substance[s] in the extract. The results support the usage of this plant in traditional medicine